![]() ![]() A poster will be presented that describes a comparative study between LOCAD-PTS analysis and existing culture-based methods onboard the ISS together with an exploratory survey of surface endotoxin throughout the ISS. This handheld device and sampling system is known as the Lab-On-a-Chip Application Development Portable Test System (LOCAD-PTS). This technique involves analysis of environmental samples with the Limulus Amebocyte Lysate (LAL) assay in a handheld device. This report describes the operation of a new culture-independent technique onboard the ISS for rapid analysis (within minutes) of endotoxin and -1, 3-glucan, found in the cell walls of gram-negative bacteria and fungi, respectively. However, the approach is also limited by the following: i) More than 95% microorganisms in the environment cannot grow on conventional growth media ii) Significant time lags occur between onboard sampling and colony visualization (3-5 days) and ground-based analysis (as long as several months) iii) Colonies are often difficult to visualize due to condensation within contact slide media plates and iv) Techniques involve growth of potentially harmful microorganisms, which must then be disposed of safely. This approach has provided a wealth of useful data and enhanced our understanding of the microbial ecology within space stations. These techniques involve growing environmental samples (cabin water, air or surfaces) on agar-type media for several days, followed by visualization of resulting colonies and return of samples to Earth for ground-based analysis. LOCAD-PTS: Operation of a New System for Microbial Monitoring Aboard the International Space Station (ISS) Microorganisms within the space stations Salyut, Mir and the International Space Station (ISS), have traditionally been monitored with culture-based techniques. ![]()
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